Modulation of dynamic aggregation in fluorogenic SNAP‐tag probes for long‐term super‐resolution imaging

The combination of super-resolution microscopy and synthetic fluorescence probes has emerged as a universal tool to monitor dynamic biological events at the nanometer scale. However, limited site-specificity fluorogenicity fluorescent make it still difficult realize long-term imaging. Herein, we introduce aggregation mediated SNAP-tag fluorogenic probe, BGAN-Aze, which can specifically bind various fusion proteins with 41-fold enhancement. equilibrium between non-fluorescent aggregate/dimer (A–D) monomer (M) BGAN-Aze acts an effective method reduce background endow capability conducting washing-free imaging intracellular extracellular proteins. Using this monitored multiple events, such MMC, mitophagy, nucleolus, growth contact filopodia. We expect that will become widely used for A–D–M be general strategy designing probes.